Rabbit Anti-Spike Protein IgG (Affinity Purified) for ELISA WB IF FC IP

Product Name: Enzyme Reagent

Host Species: Rabbit Purification Method: Affinity purification Isotype: IgG

Background The spike (S) glycoprotein of coronaviruses is a critical structural component responsible for viral entry into host cells. It consists of two functional subunits, S1 and S2. The S1 subunit contains the receptor-binding domain (RBD), which mediates recognition and binding to specific host cell receptors such as ACE2 (angiotensin-converting enzyme 2), DPP4 (dipeptidyl peptidase-4), APN (aminopeptidase N), CEACAM1 (carcinoembryonic antigen-related cell adhesion molecule 1), and sialic acid derivatives including O-acetylated sialic acid. This interaction determines host specificity and is essential for viral infectivity. The spike protein also plays a central role in eliciting neutralizing antibodies and T-cell responses, contributing to protective immunity. The S protein of SARS-CoV-2 (2019-nCoV) has been shown to bind the human ACE2 receptor, enabling infection of human respiratory epithelial cells. As a type I transmembrane glycoprotein, the spike is classified as a peplomer due to its functional clustering on the viral surface.

Applications Recommended Dilutions: - ELISA: 1:1000 – 1:5000 - Western Blotting (WB): 1:2000 – 1:10000 - Flow Cytometry (FC): 1:50 – 1:200 - Immunofluorescence / Immunochemistry (IF/ICC): 1:50 – 1:200 - Immunoprecipitation (IP): 1:50 – 1:200

Application Key: Western Blotting, Flow Cytometry, Immunofluorescence, Immunoprecipitation

Positive Control Sample: 293T cells

Storage Conditions Store at 4°C. Avoid repeated freeze-thaw cycles. Buffer Composition: PBS with 0.05% Proclin 300, pH 7.3

Company Name: Gold Medicals