Klenow Fragment (3 5 exo-) Reagent

Product Description

Gold Medicals offers Klenow Fragment (3'→ 5' exo-), a truncated form of DNA Polymerase I derived from recombinant expression systems. This enzyme retains robust polymerase activity while lacking both the 5'→ 3' exonuclease and 3'→ 5' exonuclease activities due to specific mutations (D355A, E357A) that inactivate the proofreading function.

The Klenow Fragment (3'→ 5' exo-) is ideal for applications requiring precise DNA synthesis without nucleotide degradation. It efficiently generates labeled probes using random primers and demonstrates moderate strand displacement capability, making it suitable for a range of molecular biology workflows.

Key Applications: - Random primer labeling - Sanger dideoxy DNA sequencing - Second-strand cDNA synthesis - Second-strand synthesis in site-directed mutagenesis protocols

The enzyme is produced under stringent quality control processes and is optimized for high performance and consistency in research environments. It supports reliable results in both basic research and applied molecular diagnostics.

Compliance: - Manufactured in accordance with Good Laboratory Practices (GLP) - Certified for use in research applications - Free from detectable contaminating nucleases