SARS-CoV-2 Spike S1S2 ECD Antibody Reagent Kit

Product Description: Antibody Reagent Kit

Key Features - Target Antigen: SARS-CoV-2 Spike (S) glycoprotein, comprising the full ectodomain of both S1 and S2 subunits (S-ECD), derived from HEK293 expression system - Immunogen Source: Recombinant protein expressed in HEK293 cells - Antibody Type: Rabbit IgG - Purification Method: Affinity purification - Endotoxin Level: < 1.0 EU/μg, measured by Limulus Amebocyte Lysate (LAL) assay - Formulation: Sterile filtered solution at 0.2 μm in PBS buffer with 0.1% Brij® MB-1, pH 7.4

Clinical and Functional Significance The spike glycoprotein plays a critical role in viral entry by mediating receptor binding and membrane fusion. It determines host specificity and is the primary target for neutralizing antibodies. The S1 subunit contains the receptor-binding domain (RBD), which facilitates interaction with human ACE2 receptors, enabling infection of respiratory epithelial cells. This makes the spike protein a key element in viral pathogenesis, immune response, and vaccine development. Mutations or recombination events within the RBD may enhance transmissibility and virulence across host species.

Cross-Reactivity Profile - Positive cross-reactivity demonstrated in ELISA assays with: - SARS-CoV-2 Spike S1 Protein (Cat# RP01262) - SARS-CoV-2 S1+S2 ECD(S-ECD) Protein (Cat# RP01284) - No detectable cross-reactivity in ELISA with: - MERS-CoV Spike S1 Protein

Applications Suitable for use in immunoassays including ELISA, Western blotting, and antigen capture assays. Ideal for research focused on SARS-CoV-2 spike protein interactions, neutralizing antibody detection, and comparative studies of coronavirus variants.

Compliance & Quality Assurance Manufactured under controlled conditions compliant with ISO 13485 standards. The product is free from detectable contamination and suitable for use in diagnostic and preclinical research settings.