Recombinant Human IL-1β Protein 6His Tag E coli Derived

Product Description

Gold Medicals offers Recombinant Human IL-1 beta Protein, produced using an E. coli expression system. The target protein corresponds to amino acids Ala117-Ser269 of human IL-1 beta (Accession #NP_000567.1), with an initial methionine at the N-terminus and a C-terminal 6×His tag for purification and detection.

Bioactivity

• Demonstrated in a NF-κB reporter assay using HEK293 cells, the ED50 is 1–10 pg/mL.
• Measured via cell proliferation assay with D10.G4.1 mouse helper T cells, the ED50 ranges from 0.217 to 0.870 ng/mL.
• Functional ELISA confirms binding ability: immobilized Human IL-1 beta (Catalog: RP00002) at 10 μg/mL binds biotinylated Human IL-1R1 (Catalog: RP01036) within a linear range of 0.002–1 μg/mL.

Background

Interleukin-1 beta (IL-1β or IL1B), also known as catabolin, belongs to the interleukin 1 cytokine family. It is synthesized by activated macrophages as an inactive proprotein and cleaved into its active form by caspase 1 (CASP1/ICE). This cytokine plays a central role in mediating inflammatory responses and regulates diverse cellular processes such as proliferation, differentiation, and apoptosis. In the central nervous system, IL-1β-induced upregulation of cyclooxygenase-2 (PTGS2/COX2) contributes to inflammatory pain hypersensitivity.

Reconstitution

Reconstitute the lyophilized powder to a concentration of 0.1–0.5 mg/mL using sterile distilled water. Store as directed to maintain stability and activity.